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Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.   The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens.   Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.

Dictyostelium discoideum --- Models, Theoretical --- Publication Formats --- Dictyosteliida --- Biology --- Investigative Techniques --- Biological Science Disciplines --- Publication Characteristics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Mycetozoa --- Natural Science Disciplines --- Amoebozoa --- Disciplines and Occupations --- Eukaryota --- Organisms --- Laboratory Manuals --- Models, Biological --- Dictyostelium --- Genetic Techniques --- Models, Genetic --- Genetics --- Health & Biological Sciences --- Microbiology & Immunology --- Cytogenetics --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Dictyostelid Cellular Slime Molds --- Slime Molds, Cellular --- Cellular Slime Mold --- Cellular Slime Molds --- Dictyosteliidas --- Mold, Cellular Slime --- Molds, Cellular Slime --- Slime Mold, Cellular --- Experimental Model --- Experimental Models --- Mathematical Model --- Model, Experimental --- Models (Theoretical) --- Models, Experimental --- Models, Theoretic --- Theoretical Study --- Mathematical Models --- Model (Theoretical) --- Model, Mathematical --- Model, Theoretical --- Models, Mathematical --- Studies, Theoretical --- Study, Theoretical --- Theoretical Model --- Theoretical Models --- Theoretical Studies --- Genetic Models --- Genetic Model --- Model, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Dictyostelium discoideums --- Dictyosteliums --- discoideum, Dictyostelium --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biological Model --- Biological Models --- Model, Biological --- Models, Biologic --- Biologic Model --- Biologic Models --- Model, Biologic --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- methods --- Eumycetozoa --- Eumycetozoas --- Mycetozoas --- Life sciences. --- Microbiology. --- Life Sciences. --- Eukaryotic Microbiology. --- Microbial biology --- Microorganisms --- Biosciences --- Science

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It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment.  In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants.  Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Plant growth regulators. --- Plant cellular signal transduction --- Botanical chemistry --- Growth (Plants) --- Laboratory manuals. --- Regulation. --- Plant regulators --- Publication Formats --- Biological Science Disciplines --- Growth Substances --- Natural Science Disciplines --- Physiological Effects of Drugs --- Publication Characteristics --- Pharmacologic Actions --- Disciplines and Occupations --- Chemical Actions and Uses --- Chemicals and Drugs --- Laboratory Manuals --- Plant Growth Regulators --- Physiology --- Botany --- Earth & Environmental Sciences --- Plant Physiology --- Growth Regulators, Plant --- Regulators, Plant Growth --- Phytohormones --- Plant Hormones --- Hormones, Plant --- Chemical Actions --- Actions, Chemical --- Actions, Pharmacologic --- Drug Effects on Physiology --- Drug Physiological Effects --- Effects, Drug Physiological --- Physiological Effects, Drug --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Mitogens, Endogenous --- Endogenous Mitogens --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Plant growth regulators --- Life sciences. --- Plant biochemistry. --- Plant science. --- Botany. --- Life Sciences. --- Plant Sciences. --- Plant Biochemistry. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Biosciences --- Science --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Floristic botany

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Synthetic biology, which aims to design and build organisms that serve human needs, has potential applications that range from producing biofuels to programming human behaviour. The emergence of this new form of biotechnology, however, raises a variety of ethical questions - first and foremost, whether synthetic biology is intrinsically troubling in moral terms. Is it an egregious example of scientists 'playing God'? This book takes on this key ethical question, as well as others that follow, offering a range of philosophical and political perspectives on the power of synthetic biology.

Artificial life -- Moral and ethical aspects. --- Bioengineering -- Moral and ethical aspects. --- Bioethics. --- Synthetic biology -- Moral and ethical aspects. --- Bioengineering --- Synthetic biology --- Artificial life --- Moral and ethical aspects. --- PHILOSOPHY/Ethics & Bioethics --- PHILOSOPHY/General --- BIOMEDICAL SCIENCES/General --- Life --- Biology --- Engineering --- Biological engineering --- Life science engineering --- Biomedical ethics --- Life sciences --- Life sciences ethics --- Science --- Moral and ethical aspects --- Philosophy of science --- Professional ethics. Deontology --- Biotechnology --- Bioethics --- Biological Science Disciplines --- Natural Science Disciplines --- Technology, Industry, and Agriculture --- Disciplines and Occupations --- Technology, Industry, Agriculture --- Synthetic Biology --- Health & Biological Sciences --- Biology - General --- Biologies, Synthetic --- Biology, Synthetic --- Synthetic Biologies --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Engineerings --- Bio engineering --- Biological Engineering --- Bio-Engineering --- Bio engineerings --- Bioengineerings --- Engineering, Biological --- engineering, Bio --- engineerings, Bio --- Occupations --- Technology --- Arts, Industrial --- Industrial Arts --- Cloud Computing --- Vocations --- Occupation --- Vocation --- Occupational Groups

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This book constitutes the refereed proceedings of the 5th International Conference on Social Robotics, ICSR 2013, held in Bristol, UK, in October 2013. The 55 revised full papers and 13 abstracts were carefully reviewed and selected from 108 submissions and are presented together with one invited paper. The papers cover topics such as human-robot interaction, child development and care for the elderly, as well as technical issues underlying social robotics: visual attention and processing, motor control and learning.

Engineering & Applied Sciences --- Mechanical Engineering --- Computer Science --- Mechanical Engineering - General --- Electronics --- Automation --- Computing Methodologies --- Technology --- Physics --- Information Science --- Natural Science Disciplines --- Technology, Industry, and Agriculture --- Disciplines and Occupations --- Technology, Industry, Agriculture --- Robotics --- Artificial Intelligence --- Computational Intelligence --- AI (Artificial Intelligence) --- Computer Reasoning --- Computer Vision Systems --- Knowledge Acquisition (Computer) --- Knowledge Representation (Computer) --- Machine Intelligence --- Acquisition, Knowledge (Computer) --- Computer Vision System --- Intelligence, Artificial --- Intelligence, Computational --- Intelligence, Machine --- Knowledge Representations (Computer) --- Reasoning, Computer --- Representation, Knowledge (Computer) --- System, Computer Vision --- Systems, Computer Vision --- Vision System, Computer --- Vision Systems, Computer --- Soft Robotics --- Remote Operations (Robotics) --- Telerobotics --- Operation, Remote (Robotics) --- Operations, Remote (Robotics) --- Remote Operation (Robotics) --- Robotic, Soft --- Robotics, Soft --- Soft Robotic --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Information Sciences --- Science, Information --- Sciences, Information --- Physic --- Arts, Industrial --- Industrial Arts --- High Performance Computing --- Methodologies, Computing --- Computing Methodology --- Computing, High Performance --- Methodology, Computing --- Performance Computing, High --- Automations --- Electronic --- Computer science. --- User interfaces (Computer systems). --- Artificial intelligence. --- Computers and civilization. --- Robotics. --- Automation. --- Computer Science. --- Artificial Intelligence (incl. Robotics). --- Computers and Society. --- User Interfaces and Human Computer Interaction. --- Robotics and Automation. --- Artificial intelligene --- Human-robot interaction --- HRI (Human-robot interaction) --- Robot-human interaction --- Human engineering --- Artificial intelligence --- Artificial Intelligence. --- Informatics --- Science --- AI (Artificial intelligence) --- Artificial thinking --- Electronic brains --- Intellectronics --- Intelligent machines --- Machine intelligence --- Thinking, Artificial --- Bionics --- Cognitive science --- Digital computer simulation --- Electronic data processing --- Logic machines --- Machine theory --- Self-organizing systems --- Simulation methods --- Fifth generation computers --- Neural computers --- Interfaces, User (Computer systems) --- Human-machine systems --- Human-computer interaction --- Civilization and computers --- Civilization --- Automatic factories --- Automatic production --- Computer control --- Engineering cybernetics --- Factories --- Industrial engineering --- Mechanization --- Assembly-line methods --- Automatic control --- Automatic machinery --- CAD/CAM systems

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The first textbook on research methods and methodological questions in memory studiesGBS_insertPreviewButtonPopup(['ISBN:9780748645954','ISBN:9780748645961','ISBN:9780748683475']);This guide provides students and researchers with a clear set of outlines and discussions of particular methods of research in memory studies. It offers not only expert appraisals of a range of techniques, approaches and perspectives in memory studies, but also focuses on key questions of methodology in order to help bring unity and coherence to this new field of study.Key Features:Investigates community remembering and memory in personal narrativesExplores the localisation of official national memoryAttends to painful pasts and disrupted memoryExamines vernacular remembering and personalised mediaFocuses on the production of social memory in the mediaAnalyses the dynamics of remembering in public confessions"

REFERENCE --- Research --- Memory --- Learning --- Methods --- Investigative Techniques --- Science --- Mental Processes --- Psychological Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Psychiatry and Psychology --- Disciplines and Occupations --- Research Design --- Social Sciences --- Psychology --- Methodology --- Memory. --- Research Design. --- Kollektives Gedächtnis. --- Forschungsmethode. --- Methodologie. --- Methodology. --- Data Adjustment --- Data Reporting --- Design, Experimental --- Designs, Experimental --- Error Sources --- Experimental Designs --- Matched Groups --- Methodology, Research --- Problem Formulation --- Research Methodology --- Research Proposal --- Research Technics --- Research Techniques --- Scoring Methods --- Experimental Design --- Adjustment, Data --- Adjustments, Data --- Data Adjustments --- Design, Research --- Designs, Research --- Error Source --- Formulation, Problem --- Formulations, Problem --- Group, Matched --- Groups, Matched --- Matched Group --- Method, Scoring --- Methods, Scoring --- Problem Formulations --- Proposal, Research --- Proposals, Research --- Reporting, Data --- Research Designs --- Research Proposals --- Research Technic --- Research Technique --- Scoring Method --- Source, Error --- Sources, Error --- Technic, Research --- Technics, Research --- Technique, Research --- Techniques, Research --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Psychologic Processes and Principles --- Human Information Processing --- Information Processing, Human --- Sciences --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Laboratory Research --- Research Activities --- Research and Development --- Research Priorities --- Activities, Research --- Activity, Research --- Development and Research --- Priorities, Research --- Priority, Research --- Research Activity --- Research Priority --- Research, Laboratory --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Memory Training --- Phenomenography --- Training, Memory --- Retention (Psychology) --- methods --- Research Strategy --- Research Strategies --- Strategies, Research --- Strategy, Research --- Clinical Trials Data Monitoring Committees --- Executive Function --- Psychologic Processes --- Psychological Processes --- Phenomena, Psychological --- Processes, Psychologic --- Processes, Psychological --- Psychological Phenomenas --- Psychological Processe --- Ethics, Research --- Memory Consolidation --- Education --- Intellect --- Thought and thinking --- Comprehension --- Executive functions (Neuropsychology) --- Mnemonics --- Perseveration (Psychology) --- Reproduction (Psychology) --- Techniques --- Technique